The voice of propaganda

Building on ethnographic fieldwork in Vietnam’s vibrant capital Hanoi, this article asks why attempts to use moralising public iconography as talking points with research collaborators can so often have a silencing effect on otherwise voluble interlocutors. It is proposed that these are moments of agentive silence, where the muting of a vocal self can be an act of moral will, not the crushing of agency and voice. It is therefore suggested that there can be more to a silent self than the effect of a censor’s power to control or extinguish speech, especially in contexts where state propaganda can work both visually and textually to repress as well as authorise a citizen’s expressive voice

Robust acoustic trapping and perturbation of single-cell microswimmers illuminate three-dimensional swimming and ciliary coordination

We report a label-free acoustic microfluidic method to confine single, cilia-driven swimming cells in space without limiting their rotational degrees of freedom. Our platform integrates a surface acoustic wave (SAW) actuator and bulk acoustic wave (BAW) trapping array to enable multiplexed analysis with high spatial resolution and trapping forces that are strong enough to hold individual microswimmers. The hybrid BAW/SAW acoustic tweezers employ high-efficiency mode conversion to achieve submicron image resolution while compensating for parasitic system losses to immersion oil in contact with the microfluidic chip. We use the platform to quantify cilia and cell body motion for wildtype biciliate cells, investigating effects of environmental variables like temperature and viscosity on ciliary beating, synchronization, and three-dimensional helical swimming. We confirm and expand upon the existing understanding of these phenomena, for example determining that increasing viscosity promotes asynchronous beating. Motile cilia are subcellular organelles that propel microorganisms or direct fluid and particulate flow. Thus, cilia are critical to cell survival and human health. The unicellular alg

An Anthropology of Intellectual Exchange: Interactions, Transactions and Ethics in Asia and Beyond

Dialogues, encounters and interactions through which particular ways of knowing, understanding and thinking about the world are forged lie at the centre of anthropology. Such ‘intellectual exchange’ is also central to anthropologists’ own professional practice: from their interactions with research participants and modes of pedagogy to their engagements with each other and scholars from adjacent disciplines. This collection of essays explores how such processes might best be studied cross-culturally. Foregrounding the diverse interactions, ethical reasoning, and intellectual lives of people from across the continent of Asia, the volume develops an anthropology of intellectual exchange itself

Whole-Exome Capture and Sequencing Identifies HEATR2 Mutation as a Cause of Primary Ciliary Dyskinesia

Motile cilia are essential components of the mucociliary escalator and are central to respiratory-tract host defenses. Abnormalities in these evolutionarily conserved organelles cause primary ciliary dyskinesia (PCD). Despite recent strides characterizing the ciliome and sensory ciliopathies through exploration of the phenotype-genotype associations in model organisms, the genetic bases of most cases of PCD remain elusive. We identified nine related subjects with PCD from geographically dispersed Amish communities and performed exome sequencing of two affected individuals and their unaffected parents. A single autosomal-recessive nonsynonymous missense mutation was identified in HEATR2, an uncharacterized gene that belongs to a family not previously associated with ciliary assembly or function. Airway epithelial cells isolated from PCD-affected individuals had markedly reduced HEATR2 levels, absent dynein arms, and loss of ciliary beating. MicroRNA-mediated silencing of the orthologous gene in Chlamydomonas reinhardtii resulted in absent outer dynein arms, reduced flagellar beat frequency, and decreased cell velocity. These findings were recapitulated by small hairpin RNA-mediated knockdown of HEATR2 in airway epithelial cells from unaffected donors. Moreover, immunohistochemistry studies in human airway epithelial cells showed that HEATR2 was localized to the cytoplasm and not in cilia, which suggests a role in either dynein arm transport or assembly. The identification of HEATR2 contributes to the growing number of genes associated with PCD identified in both individuals and model organisms and shows that exome sequencing in family studies facilitates the discovery of novel disease-causing gene mutations

Metabolic and immune effects of immunotherapy with proinsulin peptide in human new-onset type 1 diabetes*

Immunotherapy using short immunogenic peptides of disease-related autoantigens restores immune tolerance in preclinical disease models. We studied safety and mechanistic effects of injecting human leukocyte antigen–DR4(DRB1*0401)–restricted immunodominant proinsulin peptide intradermally every 2 or 4 weeks for 6 months in newly diagnosed type 1 diabetes patients. Treatment was well tolerated with no systemic or local hypersensitivity. Placebo subjects showed a significant decline in stimulated C-peptide (measuring insulin reserve) at 3, 6, 9, and 12 months versus baseline, whereas no significant change was seen in the 4-weekly peptide group at these time points or the 2-weekly group at 3, 6, and 9 months. The placebo group’s daily insulin use increased by 50% over 12 months but remained unchanged in the intervention groups. C-peptide retention in treated subjects was associated with proinsulin-stimulated interleukin-10 production, increased FoxP3 expression by regulatory T cells, low baseline levels of activated β cell–specific CD8 T cells, and favorable β cell stress markers (proinsulin/C-peptide ratio). Thus, proinsulin peptide immunotherapy is safe, does not accelerate decline in β cell function, and is associated with antigen-specific and nonspecific immune modulation

The voice of propaganda

Building on ethnographic fieldwork in Vietnam’s vibrant capital Hanoi, this article asks why attempts to use moralising public iconography as talking points with research collaborators can so often have a silencing effect on otherwise voluble interlocutors. It is proposed that these are moments of agentive silence, where the muting of a vocal self can be an act of moral will, not the crushing of agency and voice. It is therefore suggested that there can be more to a silent self than the effect of a censor’s power to control or extinguish speech, especially in contexts where state propaganda can work both visually and textually to repress as well as authorise a citizen’s expressive voice